Elisa Friska Romasi & Jinho Lee (2013). Development of Indole-3-Acetic Acid-Producing Escherichia coli by Functional Expression of IpdC, AspC, and Iad1. Journal of microbiology and biotechnology.
Strains
- Escherichia coli DHT36
- Escherichia coli DHT39
- Escherichia coli DH5α/pCTIP22
- Escherichia coli DH5α/pCIA41
- Escherichia coli IAA51
- Escherichia coli DH5α/pCES208
- Escherichia coli DHT39/pCES208
- Escherichia coli DHT39/pCTIP22
- Escherichia coli DHT39/pCIA41
- Escherichia coli IAA68
- Escherichia coli DH5α
Plasmids
- pCES208
- pCL1921
- pTZiad1c
- pKIP16
- pKAS12
- pKID15
- pCTIP22
- pCTAS18
- pCSAS19
- pCTID21
- pCIA41
- pCLTID23
- pKK223-3
Metabolite production
-
(indol-3-yl)acetate
-
Titer
- 3.0 g/L (Escherichia coli IAA68 in Flask)
- 1.1 g/L (Escherichia coli IAA51 in Flask)
- 0.008 g/L (Escherichia coli DH5α/pCTIP22 in Flask)
- 0.015 g/L (Escherichia coli DH5α/pCIA41 in Flask)
- 0.062 g/L (Escherichia coli DHT39/pCTIP22 in Flask)
- 0.031 g/L (Escherichia coli DHT39/pCIA41 in Flask)
- 1.8 g/L (Escherichia coli IAA68 in Flask) Substrate yield
- 0.66 mol/mol of substrate (Escherichia coli IAA51 in Flask)
-
indole-3-ethanol
-
Titer
- 0.19 g/L (Escherichia coli DH5α/pCTIP22 in Flask)
- 0.13 g/L (Escherichia coli IAA51 in Flask)
- 0.46 g/L (Escherichia coli DH5α/pCIA41 in Flask)
- 0.32 g/L (Escherichia coli DHT39/pCTIP22 in Flask)
- 0.83 g/L (Escherichia coli DHT39/pCIA41 in Flask)
- 0.032 g/L (Escherichia coli IAA68 in Flask) Substrate yield
- 1.05 mol/mol of substrate (Escherichia coli IAA68 in Flask)