Khanijou, Jasmeet Kaur; Hee, Yan Ting; Scipion, Clement P. M.; Chen, Xixian & Selvarajoo, Kumar. Systems biology approach for enhancing limonene yield by re-engineering Escherichia coli. npj Systems Biology and Applications. 2024, 10(1).
Strains
- Escherichia coli EcoCTs03
- Escherichia coli HK overexpressed
- Escherichia coli K-12 MG1655 ΔldhA
- Escherichia coli K-12 MG1655 ΔadhE
- Escherichia coli LDH KO
- Escherichia coli ALDH-ADH KO
- Escherichia coli MG1655
Plasmids
Metabolite production
-
(4S)-limonene
-
Biomass-specific titer
- 1291.7 µM/gDCW (Escherichia coli HK overexpressed in Flask)
- 1812.5 µM/gDCW (Escherichia coli HK overexpressed in Flask)
- 1312.5 µM/gDCW (Escherichia coli ALDH-ADH KO in Flask)
- 1729.2 µM/gDCW (Escherichia coli ALDH-ADH KO in Flask)
- 3791.7 µM/gDCW (Escherichia coli LDH KO in Flask)
- 2520.83 µM/gDCW (Escherichia coli LDH KO in Flask)
- 229.2 µM/gDCW (Escherichia coli EcoCTs03 in Flask)
- 229.2 µM/gDCW (Escherichia coli EcoCTs03 in Flask)