Ekaterina A. Savrasova & Nataliya V. Stoynova (2019). Application of leucine dehydrogenase Bcd from Bacillus subtilis for l-valine synthesis in Escherichia coli under microaerobic conditions. Heliyon.

Strains

• Escherichia coli MG1655 4Δ
• Escherichia coli BW25113 ΔilvE::KmR
• Escherichia coli V1
• Escherichia coli V1 cat-PL-bcd _5.7
• Escherichia coli V1 cat-PL-ilvE_5.7

Plasmids

• pUC57-bcd-Bsub
• pMW118
• pMW118-bcd

Metabolite production

• L-valine
  Titer
  • 4.1 g/L (Escherichia coli V1 cat-PL-ilvE_5.7 in Flask)
  • 9.8 g/L (Escherichia coli V1 cat-PL-ilvE_5.7 in Flask)
  • 9.1 g/L (Escherichia coli V1 cat-PL-bcd _5.7 in Flask)
  • 6.9 g/L (Escherichia coli V1 cat-PL-bcd _5.7 in Flask)