Chen, Chen; Wang, Tiantian; Ye, Pan & Li, Naiqiang. Metabolic engineering of Escherichia coli for the efficient production of 5-hydroxyvaleric acid. Process Biochemistry. 2023, 130, 625-633.
Strains
- Escherichia coli 5HV1
- Escherichia coli 5HV2
- Escherichia coli 5HV3
- Escherichia coli 5HV4
- Escherichia coli 5HV5
- Escherichia coli 5HV6
- Escherichia coli 5HV7
- Escherichia coli 5HV8
- Escherichia coli 5HV9
- Escherichia coli W3110
Plasmids
- pUC57
- pUC57-davBA
- pUC57-davBA-gabT
- UC57-davBA-gabT-yqhD
- pSTV-Plac-T7rbs-davBA-gabT-yqhD-gabP
- pSTV-Plac-T7rbs-davBA-gabT-yqhD-gabP-lysP
- pSTV-Plac-T7rbs-davBA-gabT-yqhD-gabP-lysP-hok/sok
- pCDF-Trc
- pCDF-Trc-yajO
- pCDF-Trc-yahK
- pCDF-Trc-yqhD
- pCDF-Trc-yajO-hok/sok
- pSTV-Plac-T7rbs-davBA-gabT-yqhD
- pSTV-Plac-T7rbs-davBA-gabT-yahK
- pSTV-Plac-T7rbs-davBA-gabT-yajO
- pSTV28
- pCDFDuet-1
- pTrc99A
Metabolite production
-
5-hydroxypentanoate
-
Titer
- 1.22 g/L (Escherichia coli 5HV1 in Flask)
- 0.97 g/L (Escherichia coli 5HV2 in Flask)
- 0.93 g/L (Escherichia coli 5HV3 in Flask)
- 1.87 g/L (Escherichia coli 5HV6 in Flask)
- 1.47 g/L (Escherichia coli 5HV4 in Flask)
- 1.62 g/L (Escherichia coli 5HV5 in Flask)
- 2.73 g/L (Escherichia coli 5HV8 in Flask)
- 5.59 g/L (Escherichia coli 5HV9 in Flask)
- 35.6 g/L (Escherichia coli 5HV9 in Batch)
- 2.82 g/L (Escherichia coli 5HV7 in Flask) Substrate yield
- 0.96 mol/mol of substrate (Escherichia coli 5HV9 in Flask)
- 0.92 mol/mol of substrate (Escherichia coli 5HV9 in Batch)