Xu, Daqing & Zhang, Lirong. Pathway Engineering for Phenethylamine Production in Escherichia coli. Journal of Agricultural and Food Chemistry. 2020, 68(21), 5917-5926.

Strains

• Escherichia coli AUD1
• Escherichia coli AUD2
• Escherichia coli AUD3
• Escherichia coli AUD4
• Escherichia coli AUD5
• Escherichia coli AUD6
• Escherichia coli AUD7
• Escherichia coli AUD8
• Escherichia coli AUD9
• Escherichia coli W3110

Metabolite production

• 2-phenylethylamine
  Titer
  • 2.65 g/L (Escherichia coli AUD9 in Batch)
  • 0.0 g/L (Escherichia coli W3110 in Flask)
  • 0.06 g/L (Escherichia coli AUD1 in Flask)
  • 0.15 g/L (Escherichia coli AUD2 in Flask)
  • 0.19 g/L (Escherichia coli AUD3 in Flask)
  • 0.29 g/L (Escherichia coli AUD4 in Flask)
  • 0.33 g/L (Escherichia coli AUD5 in Flask)
  • 0.4 g/L (Escherichia coli AUD8 in Flask)
  • 0.46 g/L (Escherichia coli AUD9 in Flask)
  • 38.82 g/L (Escherichia coli AUD9 in Fed-Batch)
  Substrate yield
  • 0.27 g/g of sustrate (Escherichia coli AUD9 in Batch)
  Volumetric productivity
  • 1.08 g/L/h (Escherichia coli AUD9 in Fed-Batch)
• tyramine
  Titer
  • 0.03 g/L (Escherichia coli AUD9 in Flask)