Ryo Osawa, Tomoyuki Kamide, Yasuharu Satoh, Yusuke Kawano, Iwao Ohtsu & Tohru Dairi (2018). Heterologous and High Production of Ergothioneine in Escherichia coli. Journal of Agricultural & Food Chemistry.

Strains

• Escherichia coli XL1-Blue
• Escherichia coli ET1
• Escherichia coli ET2
• Escherichia coli ET4
• Escherichia coli ET3
• Escherichia coli BW25113

Plasmids

• pQE1a-Red
• pCF1s-Red
• pET-21a
• pQE1a-MsC
• pQE1a-MsB
• pQE1a-mMsB
• pQE1a-mMsB/EcA
• pQE1a-hMsC
• pQE1a-MsE
• pQE1a-hMsE
• pAC1c-hMsC/hMsE
• pCF1s-MsD
• pACYCDuet-1

Metabolite production

• hercynine
  Titer
  • 91.0 mg/L (Escherichia coli ET1 in Flask)
  • 110.0 mg/L (Escherichia coli ET2 in Flask)
  • 73.0 mg/L (Escherichia coli ET3 in Flask)
  • 48.0 mg/L (Escherichia coli ET3 in Flask)
  • 121.0 mg/L (Escherichia coli ET4 in Flask)
• γ-L-glutamyl-S-(hercyn-2-yl)-L-cysteine S-oxide
  Titer
  • 24.0 mg/L (Escherichia coli ET2 in Flask)
  • 1.0 mg/L (Escherichia coli ET4 in Flask)
• S-(hercyn-2-yl)-L-cysteine S-oxide
  Titer
  • 36.0 mg/L (Escherichia coli ET2 in Flask)
  • 10.0 mg/L (Escherichia coli ET3 in Flask)
  • 9.0 mg/L (Escherichia coli ET3 in Flask)
  • 9.0 mg/L (Escherichia coli ET4 in Flask)
• ergothioneine
  Titer
  • 19.0 mg/L (Escherichia coli ET3 in Flask)
  • 24.0 mg/L (Escherichia coli ET3 in Flask)
  • 17.0 mg/L (Escherichia coli ET4 in Flask)