Daoyi Guo, Sijia Kong, Xu Chu, Xun Li & Hong Pan (2019). De Novo Biosynthesis of Indole-3-acetic Acid in Engineered Escherichia coli. Journal of Agricultural & Food Chemistry.

Strains

• Escherichia coli RARE
• Escherichia coli DG120
• Escherichia coli DG121
• Escherichia coli DG122

Plasmids

• pDG-AldH
• pDG9
• pDG11
• pDG30
• pDG31
• pDG32
• pDG33
• pDG34
• pDG35

Metabolite production

• (indol-3-yl)acetate
  Titer
  • 387.0 mg/L (Escherichia coli DG121 in Flask)
  • 744.0 mg/L (Escherichia coli DG122 in Flask)
  Substrate yield
  • 0.9 mol/mol of substrate (Escherichia coli DG121 in Flask)