Hou, Yanan; Liu, Xue; Li, Shilin; Zhang, Xue; Yu, Sili & Zhao, Guang-Rong. Metabolic Engineering of Escherichia coli for de Novo Production of Betaxanthins. Journal of Agricultural and Food Chemistry. 2020, 68(31), 8370-8380.

Strains

• Escherichia coli BAK11(DE3)
• Escherichia coli BAK10(DE3)
• Escherichia coli BTA1
• Escherichia coli BTA2
• Escherichia coli BTA3
• Escherichia coli BTA4
• Escherichia coli BTA5
• Escherichia coli BTA7
• Escherichia coli BTA6
• Escherichia coli BTA8
• Escherichia coli BTA9
• Escherichia coli BHS1
• Escherichia coli BHS2
• Escherichia coli BHS3
• Escherichia coli BAK10
• Escherichia coli BAK11
• Escherichia coli BW25113

Plasmids

• pRSFDuet-1
• pETDuet-1
• pYSL1
• pYSL2
• pYSL3
• pHYN1
• pHYN2
• pHYN3

Metabolite production

• L-histidine
  Titer
  • 347.53 mg/L (Escherichia coli BHS2 in Flask)
  • 0.0 mg/L (Escherichia coli BW25113 in Flask)
  • 64.18 mg/L (Escherichia coli BHS1 in Flask)
  • 422.6 mg/L (Escherichia coli BHS3 in Flask)