Fan, Yucheng; Wei, Zijia; Zhang, Yuhua & Duan, Xuguo. Enhancing L-asparagine Production Through In Vivo ATP Regeneration System Utilizing Glucose Metabolism of Escherichia coli. Applied Biochemistry and Biotechnology. 2024, 196(12), 8685-8699.

Strains

• Escherichia coli BL21(DE3)/pET24a-Asn-Ec
• Escherichia coli BL21(DE3)

Plasmids

• pET-24a(+)
• pET24a/AsnA-Ec

Metabolite production

• L-asparagine
  Titer
  • 38.49 g/L (Escherichia coli BL21(DE3)/pET24a-Asn-Ec in Fed-Batch)
  • 2.7 g/L (Escherichia coli BL21(DE3)/pET24a-Asn-Ec in Flask)
  Substrate yield
  • 0.2916 mol/mol of substrate (Escherichia coli BL21(DE3)/pET24a-Asn-Ec in Fed-Batch)