Natthawut Wiriyathanawudhiwong, Iwao Ohtsu, Zhao-Di Li, Hirotada Mori & Hiroshi Takagi (2008). The outer membrane TolC is involved in cysteine tolerance and overproduction in Escherichia coli. Applied Microbiology and Biotechnology.

Strains

• Escherichia coli JW0452
• Escherichia coli JW0451
• Escherichia coli JW3233
• Escherichia coli JW3234
• Escherichia coli JW2660
• Escherichia coli JW2661
• Escherichia coli JW0862
• Escherichia coli JW0863
• Escherichia coli JW0940
• Escherichia coli JW2203
• Escherichia coli JW0912
• Escherichia coli JW0554
• Escherichia coli JW0799
• Escherichia coli JW5503
• Escherichia coli JW3832
• Escherichia coli MG1655ΔtnaA
• Escherichia coli MG1655ΔtnaA/pDES
• Escherichia coli MG1655/pCEM256I/pYdeD
• Escherichia coli MG1655ΔtnaA/pDES/pLSTolC
• Escherichia coli MG1655/pCEM256I/pYdeD/pLSTolC
• Escherichia coli MG1655/pBluescript II SK(+)/pSTV29/pLS19

Plasmids

• pDES
• pACYC184
• pSTV29
• pYdeD
• pBluescriptII KS(+)
• pCEM256I
• pMW219
• pLS219
• pLSTolC
• pCA24N
• pOmpA
• pOmpC
• pOmpF
• pOmpT
• pOmpX
• pTolC
• pDsbA
• pUC118
• pUCacrAB
• pUCacrEF
• pUCemrAB
• pUCmacAB

Metabolite production

• L-cysteine
  Titer
  • 70.0 mg/L (Escherichia coli MG1655/pCEM256I/pYdeD in Flask)
  • 958.0 mg/L (Escherichia coli MG1655ΔtnaA/pDES in Flask)
  • 1127.0 mg/L (Escherichia coli MG1655ΔtnaA/pDES/pLSTolC in Flask)
  • 119.0 mg/L (Escherichia coli MG1655/pCEM256I/pYdeD/pLSTolC in Flask)
  • 0.0 mg/L (Escherichia coli MG1655/pBluescript II SK(+)/pSTV29/pLS19 in Flask)