Lim, Jae Hyung; Seo, Sang Woo; Kim, Se Yeon & Jung, Gyoo Yeol (2013). Refactoring redox cofactor regeneration for high-yield biocatalysis of glucose to butyric acid in Escherichia coli. Bioresource Technology, 135, 568-573.

Strains

• Escherichia coli W3110
• Escherichia coli JHL08
• Escherichia coli JHL58
• Escherichia coli JHL29
• Escherichia coli JHL30
• Escherichia coli JHL25
• Escherichia coli JHL26

Plasmids

• pCDF-SP
• pACYC-SP
• pBASP

Metabolite production

• butanoate
  Titer
  • 4.35 g/L (Escherichia coli JHL26 in Flask)
  • 0.8 g/L (Escherichia coli JHL30 in Flask)
  • 0.29 g/L (Escherichia coli JHL29 in Flask)
  Substrate yield
  • 0.489 g/g of sustrate (Escherichia coli JHL26 in Flask)
  Volumetric productivity
  • 0.181 g/L/h (Escherichia coli JHL26 in Flask)