Zhu, Saijie; Wu, Junjun; Du, Guocheng; Zhou, Jingwen & Chen, Jian (2014). Efficient Synthesis of Eriodictyol from l -Tyrosine in Escherichia coli. Applied and Environmental Microbiology, 80(10), 3072-3080.

Strains

• Escherichia coli BL21(DE3)
• Escherichia coli BA
• Escherichia coli BAtF
• Escherichia coli BAtC
• Escherichia coli BAtFC
• Escherichia coli BM1
• Escherichia coli BM2
• Escherichia coli BM3
• Escherichia coli BM4
• Escherichia coli BM
• Escherichia coli BF1
• Escherichia coli BF2
• Escherichia coli BMF1
• Escherichia coli BMF2

Plasmids

• pRSFDuet-1
• pRSF-acs
• pCDF-TAL-4CL
• pETDuet-1
• pET-CHS-CHI
• pRSF-ACC
• pRSF-acs-ACC
• pACYC-acs-ACC
• pACYCDuet-1
• pACYC-tF3′H-tCPR
• pACYC-tF3′H
• pACYC- tCPR
• pRSF-tF3′H-tCPR

Metabolite production

• (2S)-eriodictyol
  Titer
  • 5.7 mg/L (Escherichia coli BMF2 in Flask)
  • 107.0 mg/L (Escherichia coli BMF2 in Flask)
  • 86.4 mg/L (Escherichia coli BMF1 in Flask)
  • 35.2 mg/L (Escherichia coli BF1 in Flask)
  • 42.6 mg/L (Escherichia coli BF2 in Flask)