Zhao, Jing; Li, Qingyan; Sun, Tao; Zhu, Xinna; Xu, Hongtao; Tang, Jinlei; Zhang, Xueli & Ma, Yanhe (2013). Engineering central metabolic modules of Escherichia coli for improving β-carotene production. Metabolic Engineering, 17, 42-50.

Strains

• Escherichia coli Crooks
• Escherichia coli QL001
• Escherichia coli QL002
• Escherichia coli QL105
• Escherichia coli Dxs37-Idi46
• Escherichia coli CAR005
• Escherichia coli CAR001
• Escherichia coli dxs37
• Escherichia coli CAR002
• Escherichia coli CAR004
• Escherichia coli ATCC 8739/ACYC184-M-crt
• Escherichia coli dxs64
• Escherichia coli dxs93
• Escherichia coli Dxs37-Idi30
• Escherichia coli Dxs37-Idi37
• Escherichia coli QL105/ACYC184-M-crt
• Escherichia coli dxs37/ACYC184-M-crt
• Escherichia coli Dxs37-Idi46/ACYC184-M-crt
• Escherichia coli CAR003

Plasmids

• pACYC184-M
• ACYC184-M-crt

Metabolite production

• β-carotene
  Biomass-specific yield
  • 59.88 mg/g DCW (Escherichia coli CAR005 in Fed-Batch)
  • 30.17 mg/g DCW (Escherichia coli CAR005 in Flask)
  • 0.41 mg/g DCW (Escherichia coli QL002 in Flask)
  • 1.53 mg/g DCW (Escherichia coli QL105 in Flask)
  • 3.56 mg/g DCW (Escherichia coli dxs37 in Flask)
  • 5.33 mg/g DCW (Escherichia coli Dxs37-Idi46 in Flask)
  • 18.4 mg/g DCW (Escherichia coli CAR001 in Flask)
  • 23.77 mg/g DCW (Escherichia coli CAR002 in Flask)
  • 27.23 mg/g DCW (Escherichia coli CAR004 in Flask)
  • 0.56 mg/g DCW (Escherichia coli ATCC 8739/ACYC184-M-crt in Flask)
  • 2.8 mg/g DCW (Escherichia coli dxs93 in Flask)
  • 3.05 mg/g DCW (Escherichia coli dxs64 in Flask)
  • 4.32 mg/g DCW (Escherichia coli Dxs37-Idi37 in Flask)
  • 4.07 mg/g DCW (Escherichia coli Dxs37-Idi30 in Flask)
  • 2.54 mg/g DCW (Escherichia coli QL105/ACYC184-M-crt in Flask)
  • 6.35 mg/g DCW (Escherichia coli dxs37/ACYC184-M-crt in Flask)
  • 12.44 mg/g DCW (Escherichia coli Dxs37-Idi46/ACYC184-M-crt in Flask)
  • 26.23 mg/g DCW (Escherichia coli CAR003 in Flask)
  Titer
  • 2.1 g/L (Escherichia coli CAR005 in Fed-Batch)
  • 0.87 mg/L (Escherichia coli ATCC 8739/ACYC184-M-crt in Flask)
  • 0.59 mg/L (Escherichia coli QL002 in Flask)
  • 2.17 mg/L (Escherichia coli QL105 in Flask)
  • 3.55 mg/L (Escherichia coli dxs93 in Flask)
  • 3.66 mg/L (Escherichia coli dxs64 in Flask)
  • 7.58 mg/L (Escherichia coli Dxs37-Idi46 in Flask)
  • 6.3 mg/L (Escherichia coli Dxs37-Idi37 in Flask)
  • 6.09 mg/L (Escherichia coli Dxs37-Idi30 in Flask)
  • 25.67 mg/L (Escherichia coli CAR001 in Flask)
  • 33.4 mg/L (Escherichia coli CAR002 in Flask)
  • 34.46 mg/L (Escherichia coli CAR003 in Flask)
  • 38.67 mg/L (Escherichia coli CAR004 in Flask)
  • 39.03 mg/L (Escherichia coli CAR005 in Flask)