Dashuai Li, Qiang Zhang, Zhijiang Zhou, Fanglong Zhao & Wenyu Lu (2016). Heterologous biosynthesis of triterpenoid dammarenediol-II in engineered Escherichia coli. Biotechnology Letters.

Strains

• Escherichia coli BL21(DE3)
• Escherichia coli DH5α
• Escherichia coli SQ1
• Escherichia coli OSQ1
• Escherichia coli OSQ2
• Escherichia coli OSQ3
• Escherichia coli OSQ4
• Escherichia coli OSQ5
• Escherichia coli OSQ6
• Escherichia coli OSQ7
• Escherichia coli OSQ8
• Escherichia coli OSQ9
• Escherichia coli DS1
• Escherichia coli DS2

Plasmids

• pET-28a(+)
• pCDFDuet-1
• pSQ1
• pSQ2
• pOSQ1
• pOSQ2
• pOSQ3
• pOSQ4
• pOSQ5
• pOSQ6
• pOSQ7
• pOSQ8
• pOSQ9
• pDS

Metabolite production

• dammarenediol II
  Titer
  • 8.63 mg/L (Escherichia coli DS1 in Flask)