Ming-Yue Fang, Chong Zhang, Song Yang, Jin-Yu Cui, Pei-Xia Jiang, Kai Lou, Masaaki Wachi & Xin-Hui Xing (2015). High crude violacein production from glucose by Escherichia coli engineered with interactive control of tryptophan pathway and violacein biosynthetic pathway. Microbial Cell Factories.
Strains
- Escherichia coli B8
- Escherichia coli B7
- Escherichia coli B6
- Escherichia coli B5
- Escherichia coli B4
- Escherichia coli B3
- Escherichia coli B2
- Escherichia coli B2/pED + pVio
- Escherichia coli B2/pED
- Escherichia coli B1/pED
- Escherichia coli B3/pED
- Escherichia coli B4/pED
- Escherichia coli B5/pED
- Escherichia coli B6/pED
- Escherichia coli B7/pED
- Escherichia coli B8/pED
- Escherichia coli B1/pED + pVio
- Escherichia coli B3/pED + pVio
- Escherichia coli B4/pED + pVio
- Escherichia coli B5/pED + pVio
- Escherichia coli B1/pVio
- Escherichia coli B6/pED + pVio
- Escherichia coli B7/pED + pVio
- Escherichia coli B8/pED + pVio
- Escherichia coli BL21(DE3)
Plasmids
Metabolite production
-
violacein
-
Volumetric productivity
- 36.0 mg/L/h (Escherichia coli B2/pED + pVio in Fed-Batch) Titer
- 1.75 g/L (Escherichia coli B2/pED + pVio in Fed-Batch)
- 0.153 g/L (Escherichia coli B1/pVio in Flask)
- 0.532 g/L (Escherichia coli B1/pED + pVio in Flask)
- 0.603 g/L (Escherichia coli B2/pED + pVio in Flask)
- 0.5557 g/L (Escherichia coli B3/pED + pVio in Flask)
- 0.5824 g/L (Escherichia coli B4/pED + pVio in Flask)
- 0.5438 g/L (Escherichia coli B5/pED + pVio in Flask)
- 0.5319 g/L (Escherichia coli B6/pED + pVio in Flask)
- 0.5646 g/L (Escherichia coli B7/pED + pVio in Flask)
- 0.5587 g/L (Escherichia coli B8/pED + pVio in Flask) Substrate yield
- 0.116 g/g of sustrate (Escherichia coli B2/pED + pVio in Flask)
-
L-tryptophan
-
Titer
- 0.18 g/L (Escherichia coli B8/pED in Flask)
- 0.032 g/L (Escherichia coli B7/pED in Flask)
- 0.175 g/L (Escherichia coli B6/pED in Flask)
- 0.0673 g/L (Escherichia coli B5/pED in Flask)
- 0.036 g/L (Escherichia coli B4/pED in Flask)
- 0.1115 g/L (Escherichia coli B3/pED in Flask)
- 0.12 g/L (Escherichia coli B2/pED in Flask)
- 0.0024 g/L (Escherichia coli B1/pED in Flask)