Inactivation or deletion of the nagB gene is a key genetic engineering strategy employed to prevent the degradation of amino sugar intermediates. Blocking the glucosamine-6-phosphate deaminase activity prevents the conversion of glucosamine-6-phosphate into fructose-6-phosphate, thereby conserving the precursor pool and channeling metabolic flux toward the high-yield production of target amino sugars, such as glucosamine and N-acetylglucosamine [220].
nagB
Escherichia coli
Sequence
ATGAGACTGATCCCCCTGACTACCGCTGAACAGGTCGGCAAATGGGCTGCTCGCCATATCGTCAATCGTATCAATGCGTTCAAACCGACTGCCGATCGTCCGTTTGTACTGGGCCTGCCGACTGGCGGCACGCCGATGACCACCTATAAAGCGTTAGTCGAAATGCATAAAGCAGGCCAGGTCAGCTTTAAGCACGTTGTCACCTTCAACATGGACGAATATGTCGGTCTGCCGAAAGAGCATCCGGAAAGCTACTACAGCTTTATGCACCGTAATTTCTTCGATCACGTTGATATTCCAGCAGAAAACATCAACCTTCTCAACGGCAACGCCCCGGATATCGACGCCGAGTGCCGCCAGTATGAAGAAAAAATCCGTTCTTACGGAAAAATTCATCTGTTTATGGGCGGTGTAGGTAACGACGGTCATATTGCATTTAACGAACCGGCGTCTTCTCTGGCTTCTCGTACTCGTATCAAAACCCTGACTCATGACACTCGCGTCGCAAACTCTCGTTTCTTTGATAACGATGTTAATCAGGTGCCAAAATATGCCCTGACTGTCGGTGTTGGTACACTGCTGGATGCCGAAGAAGTGATGATTCTGGTGCTGGGTAGCCAGAAAGCACTGGCGCTGCAGGCCGCCGTTGAAGGTTGCGTGAACCATATGTGGACCATCAGCTGTCTGCAACTGCATCCGAAAGCGATCATGGTGTGCGATGAACCTTCCACCATGGAGCTGAAAGTTAAGACTTTAAGATATTTCAATGAATTAGAAGCAGAAAATATCAAAGGTCTGTAA
Inactivation or deletion of the nagB gene is a key genetic engineering strategy employed to prevent the degradation of amino sugar intermediates. Blocking the glucosamine-6-phosphate deaminase activity prevents the conversion of glucosamine-6-phosphate into fructose-6-phosphate, thereby conserving the precursor pool and channeling metabolic flux toward the high-yield production of target amino sugars, such as glucosamine and N-acetylglucosamine [220].
| Gene size: | |
| Protein size: | |
| Reactions | R928 |
| Compounds affected | N-acetyl-β-D-glucosamine and D-glucosamine |
Databases
| EraGene: | 2111465 |
|---|---|
| KEGG: | eco:b0678 |