pEL12

Ancestors

All plasmids

pEL12

Main features

P_LlacO₁::atoB_Ec-crt_CA-hbd_CA, ColE1 ori

Selection marker:

Ampicillin

Modifications

Gene	Organism	Modification
*atoB*	Escherichia coli	Overexpression
*crt*	Clostridium acetobutylicum	Overexpression
*hbd*	Clostridium acetobutylicum	Overexpression

References

Shen, Claire R.; Lan, Ethan I.; Dekishima, Yasumasa; Baez, Antonino; Cho, Kwang Myung & Liao, James C. (2011). Driving Forces Enable High-Titer Anaerobic 1-Butanol Synthesis in Escherichia coli. Applied and Environmental Microbiology, 77(9), 2905-2915.