pIM11

Ancestors

All plasmids

pIM11

Main features

P_LlacO₁::bcd_CA-etfA_CA-etfB_CA, Cola ori

Selection marker:

Kanamycin

Modifications

Gene	Organism	Modification
*bcd*	Clostridium acetobutylicum	Overexpression
*etfA*	Clostridium acetobutylicum	Overexpression
*etfB*	Clostridium acetobutylicum	Overexpression

References

Shen, Claire R.; Lan, Ethan I.; Dekishima, Yasumasa; Baez, Antonino; Cho, Kwang Myung & Liao, James C. (2011). Driving Forces Enable High-Titer Anaerobic 1-Butanol Synthesis in Escherichia coli. Applied and Environmental Microbiology, 77(9), 2905-2915.