pAL655

Ancestors

All plasmids

pAL655

Main features

p15A ori, P_LtetO₁:bglC, P_LlacO₁:alsS-ilvCD

Selection marker:

Chloramphenicol

Modifications

Gene	Organism	Modification
*bglC*	Thermobifida fusca	Overexpression
*alsS*	Bacillus subtilis	Overexpression
*ilvC*	Escherichia coli	Overexpression
*ilvD*	Escherichia coli	Overexpression

References

Desai, Shuchi H.; Rabinovitch-Deere, Christine A.; Tashiro, Yohei & Atsumi, Shota (2014). Isobutanol production from cellobiose in Escherichia coli. Applied Microbiology and Biotechnology, 98(8), 3727-3736.