pTY12

Ancestors

All plasmids

pTac15K

pBK1

pTY12

Main features

P_tac, aroG^fbr-aroL, P_trc, tyrA^fbr

Selection marker:

Kanamycin

Modifications

Gene	Organism	Modification
*aroG*	Escherichia coli	Feedback insensitive
*aroL*	Escherichia coli	Overexpression
*tyrA*	Escherichia coli	Feedback insensitive

Strains harboring plasmid pTY12

Escherichia coli BTY0.12

Escherichia coli BTY1.12

References

Byoungjin Kim, Robert Binkley, Hyun Uk Kim & Sang Yup Lee (2018). Metabolic engineering of Escherichia coli for the enhanced production of l‐tyrosine. Biotechnology & Bioengineering.