pCS-trpE^fbrtrpG-serA*-prsA-A95T

Ancestors

All plasmids

pCS27

pCS-serA*

pCS-serA*-prsA-A95T

pCS-trpE^fbrtrpG-serA*-prsA-A95T

Main features

P_LlacO1-trpE^fbrtrpG, P_LlacO1-serA*-prsA-A95T

Selection marker:

Kanamycin

Modifications

Gene	Organism	Modification
*trpE*	Escherichia coli	Feedback insensitive
*trpD*	Escherichia coli	Overexpression
*serA*	Escherichia coli	Feedback insensitive
*prsA*	Escherichia coli	Feedback insensitive

Strains harboring plasmid pCS-trpE^fbrtrpG-serA*-prsA-A95T

Escherichia coli TP1

Escherichia coli TP2

Escherichia coli TP3

References

Jian Wang, Ruihua Zhang, Yan Zhang, Yaping Yang, Yuheng Lin & Yajun Yan (2019). Developing a pyruvate-driven metabolic scenario for growth-coupled microbial production. Metabolic Engineering.