pSV-709

Ancestors

All plasmids

pBR322

pSV-709

Main features

aroG^fbr trpE^fbrDCBA serA

Selection marker:

Ampicillin

Tetracycline

Modifications

Gene	Organism	Modification
*serA*	Escherichia coli	Overexpression
*trpA*	Escherichia coli	Overexpression
*trpB*	Escherichia coli	Overexpression
*trpC*	Escherichia coli	Overexpression
*trpD*	Escherichia coli	Overexpression
*aroG*	Escherichia coli	Feedback insensitive
*trpE*	Escherichia coli	Feedback insensitive

Strains harboring plasmid pSV-709

References

Jian Wang, Li-Kun Cheng, Jing Wang, Qian Liu, Tong Shen & Ning Chen (2013). Genetic engineering of Escherichia coli to enhance production of l-tryptophan. Applied Microbiology and Biotechnology.
Tong Shen, Qing Liu, Xixian Xie, Qingyang Xu & Ning Chen (2012). Improved Production of Tryptophan in Genetically Engineered Escherichia coli with TktA and PpsA Overexpression. BioMed Research International.