Escherichia coli TRTH
Ancestors
Derived strains
- Escherichia coli TRTH ΔaroP
- Escherichia coli TRTH-Y
- Escherichia coli TRJH/ΔPB
- Escherichia coli TRTHΔpta
- Escherichia coli TRTHB
- Escherichia coli TRTHA
- Escherichia coli TRTHT
- Escherichia coli TRTHAT
- Escherichia coli T03
Genotype with respect to parental
trpEDCBA+TetR, ΔtnaA
Genotype with respect to wild type
F+ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | trpEDCBA+TetR, ΔtnaABars (|) indicate differences between strains.
Modifications
Gene | Organism | Modification |
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Silencing
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Production
Metabolites | Heterologous | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
---|---|---|---|---|---|---|---|---|
L-tryptophan | Titer | 46.8 g/L | 51.2 g/L | Glucose | 38 h | Fed-Batch | [ 196 ] | |
L-tryptophan | Substrate yield | 17.2 % | 51.2 g/L | Glucose | 38 h | Fed-Batch | [ 196 ] | |
acetate | Titer | 1.19 g/L | 51.2 g/L | Glucose | 38 h | Fed-Batch | [ 196 ] | |
L-glutamate | Titer | 3.28 g/L | 51.2 g/L | Glucose | 38 h | Fed-Batch | [ 196 ] |
* Inferred from plots using RetroPlot.
References
- Qian Liu, Yongsong Cheng, Xixian Xie, Qingyang Xu & Ning Chen (2012). Modification of tryptophan transport system and its impact on production of l-tryptophan in Escherichia coli. Bioresource Technology.
- Wang, J., Huang, J., Shi, J. et al. Fermentation characterization of an L-tryptophan producing Escherichia coli strain with inactivated phosphotransacetylase. Ann Microbiol 63, 1219–1224 (2013).
- Da Xu, Zhen Zhang, Ziqiang Liu & Qingyang Xu (2019). Using enzymatic hydrolyzate as new nitrogen source for L-tryptophan fermentation by E.coli. Bioengineered.
- Chunguang Zhao, LiKun Cheng, Jian Wang, Zhiqiang Shen & Ning Chen (2015). Impact of deletion of the genes encoding acetate kinase on production of L-tryptophan by Escherichia coli. Annals of Microbiology.