Escherichia coli JCL198

Ancestors

Genotype with respect to wild type

F⁺ (λ) | IacI^q rrnB_T14 ∆lacZ_WJ16 hsdR514 ∆araBAD_AH33 ∆rhaBAD_LD78 | F' [traD36, proAB⁺, lacI^q ZDM15] | Plasmid pJCL60 added | Plasmid pJCL50 added

Bars (|) indicate differences between strains.

Plasmids

pJCL50 (P_LlacO₁::thl(CA)-adhE2(CA))

pJCL60 (P_LlacO₁::crt(CA)-bcd(CA)-etfAB(CA)-hbd(CA))

Modifications

Origin	Gene	Organism	Modification
pJCL50	thlA	Clostridium acetobutylicum	Overexpression
pJCL50	adhE	Clostridium acetobutylicum	Overexpression
pJCL60	crt	Clostridium acetobutylicum	Overexpression
pJCL60	bcd	Clostridium acetobutylicum	Overexpression
pJCL60	etfA	Clostridium acetobutylicum	Overexpression
pJCL60	etfB	Clostridium acetobutylicum	Overexpression
pJCL60	hbd	Clostridium acetobutylicum	Overexpression

Production

Metabolites	Production type	Production	Carbon source	Scale	Ref.
butan-1-ol	Titer	13.9 mg/L	Glucose	Flask	[90]
butan-1-ol	Titer	19.0 mg/L	Glucose	Flask	[90]
butan-1-ol	Titer	10.0 mg/L	Glucose	Flask	[90]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Atsumi, Shota; Cann, Anthony F.; Connor, Michael R.; Shen, Claire R.; Smith, Kevin M.; Brynildsen, Mark P.; Chou, Katherine J.Y.; Hanai, Taizo & Liao, James C. (2008). Metabolic engineering of Escherichia coli for 1-butanol production. Metabolic Engineering, 10(6), 305-311.