Escherichia coli BL21(DE3)/pZYB3

Ancestors

All strains

Escherichia coli B

Escherichia coli BL21

Escherichia coli BL21(DE3)

Escherichia coli BL21(DE3)/pZYB3

Genotype with respect to wild type

F⁻ dcm gal ompT hsdS(r_B^- m_B^-) | F⁻ ompT hsdS_B (r_B⁻m_B⁻) gal dcm λ(DE3) | Plasmid pZYB3 added

Bars (|) indicate differences between strains.

Plasmids

pZYB3 (ccr_Sco)

Modifications

Origin	Gene	Organism	Modification
pZYB3	ccr	Streptomyces collinus Tü 1892	Overexpression

References

Kimberlee K. Wallace, Zhuo-Yao Bao, Hong Dai, Russell Digate, Gregory Schuler, Marilyn K. Speedie & Kevin A. Reynolds (1995). Purification of Crotonyl-CoA Reductase from Streptomyces collinus and Cloning, Sequencing and Expression of the Corresponding Gene in Escherichia coli. European Journal of Biochemistry.