Escherichia coli MA5

Ancestors

All strains

Escherichia coli K12

Escherichia coli ATCC 31884

Escherichia coli QH4

Escherichia coli JW1

Escherichia coli JW2

Escherichia coli JW3

Escherichia coli MA5

Genotype with respect to wild type

F⁺ (λ) | aroH367, tyrR366, tna-2, lacY5, aroF394^fbr, malT384, pheA101^fbr, pheO352, aroG397^fbr | ΔpheLA ΔtyrA | ΔpykA ΔpykF | ΔgldA | ΔmaeB | Plasmid pZE-PAPC added | Plasmid pCS-trpE^fbrG-APTA added

Bars (|) indicate differences between strains.

Plasmids

pCS-trpE^fbrG-APTA (trpE^fbr(S40R) trpG(N-terminal domain of trpD) aroL ppsA tktA aroG^fbr(D146N))

pZE-PAPC (antABC_Pa, catA_Pp)

Production

Metabolites	Heterologous	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
cis,cis-muconate		Titer	0.71 g/L		Glycerol	72 h	Flask	[ 205 ]

* Inferred from plots using RetroPlot.

References

Jian Wang, Ruihua Zhang, Yan Zhang, Yaping Yang, Yuheng Lin & Yajun Yan (2019). Developing a pyruvate-driven metabolic scenario for growth-coupled microbial production. Metabolic Engineering.