Escherichia coli CWF811

Ancestors

Derived strains

Genotype with respect to parental

ΔlacI


Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔiclR | ΔfumC | ΔfumA | ΔfumB | Plasmid pTac15kppc added | ΔarcA | ΔptsG | ΔaspA | ΔlacI

Bars (|) indicate differences between strains.


Plasmids

Modifications
Gene Organism Modification
Silencing

Production
Metabolites Heterologous Production type Production Biomass Carbon source Time Scale Ref.
fumarate Titer 6.25 g/L Glucose 24 h Flask [ 210 ]
fumarate Substrate yield 0.417 g/g of sustrate Glucose 24 h Flask [ 210 ]
fumarate Titer 8.23 g/L Glucose 27 h Batch [ 210 ]
fumarate Substrate yield 0.415 g/g of sustrate Glucose 27 h Batch [ 210 ]
fumarate Volumetric productivity 0.305 g/L/h Glucose 27 h Batch [ 210 ]
fumarate Specific production rate 0.201 g/g DCW/h Glucose 27 h Batch [ 210 ]
acetate Titer 2.0 g/L Glucose 27 h Batch [ 210 ]
acetate Substrate yield 0.1 g/g of sustrate Glucose 27 h Batch [ 210 ]
fumarate Titer 26.2 g/L Glucose 104 h Fed-Batch [ 210 ]
fumarate Substrate yield 0.361 g/g of sustrate Glucose 104 h Fed-Batch [ 210 ]
fumarate Volumetric productivity 0.252 g/L/h Glucose 104 h Fed-Batch [ 210 ]
acetate Titer 9.55 g/L Glucose 104 h Fed-Batch [ 210 ]
acetate Substrate yield 0.132 g/g of sustrate Glucose 104 h Fed-Batch [ 210 ]

* Inferred from plots using RetroPlot.


References