Escherichia coli BW25113
Ancestors
Derived strains
- Escherichia coli JW2944
- Escherichia coli BW25113 (pTadhB-pdc)
- Escherichia coli AQ02
- Escherichia coli AQ04
- Escherichia coli AQ09
- Escherichia coli N00
- Escherichia coli BW25113/F'
- Escherichia coli BW25113-pGLY-1
- Escherichia coli EXY-1
- Escherichia coli BW25113 ∆aceK
- Escherichia coli BW25113 ∆aceA
- Escherichia coli BW25113 ∆aceAK
- Escherichia coli BW25113 ∆caiA
- Escherichia coli BW25113 ∆pcaiF caiF-p8
- Escherichia coli BW25113 ∆pcai cai-p37
- Escherichia coli BW25113 ∆aceK ∆pcai cai-p37
- Escherichia coli BW25113 ∆aceK ∆caiA ∆pcai cai-p37
Genotype with respect to parental
IacIq rrnBT14 ∆lacZWJ16 hsdR514 ∆araBADAH33 ∆rhaBADLD78
Genotype with respect to wild type
F+ (λ) | IacIq rrnBT14 ∆lacZWJ16 hsdR514 ∆araBADAH33 ∆rhaBADLD78Bars (|) indicate differences between strains.
Production
Metabolites | Heterologous | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
---|---|---|---|---|---|---|---|---|
ethanol | Titer | 0.2 g/L | Glucose | 18 h | Flask | [ 143 ] | ||
L-carnitine | Titer | 14.23 mM * | Glucose | Flask | [ 241 ] | |||
L-carnitine | Titer | 16.62 mM * | Glucose | Flask | [ 241 ] | |||
L-carnitine | Titer | 0.0 mM * | Glucose | Flask | [ 241 ] | |||
L-carnitine | Titer | 0.0 mM * | Glucose | Flask | [ 241 ] |
* Inferred from plots using RetroPlot.
References
- Tomoya Baba, Takeshi Ara, Miki Hasegawa, Yuki Takai, Yoshiko Okumura, Miki Baba, Kirill A Datsenko, Masaru Tomita, Barry L Wanner & Hirotada Mori (2006). Construction of Escherichia coli K‐12 in‐frame, single‐gene knockout mutants: the Keio collection. Molecular Systems Biology.
- Fithriani, Prayoga Suryadarma & Djumali Mangunwidjaja (2015). Metabolic Engineering of Escherichia coli Cells for Ethanol Production under Aerobic Conditions. Procedia Chemistry.
- Zhu J, Yang W, Wang B, Liu Q, Zhong X, Gao Q, Liu J, Huang J, Lin B, Tao Y. Metabolic engineering of Escherichia coli for efficient production of L-alanyl-L-glutamine. Microb Cell Fact. 2020 Jun 11;19(1):129.
- Shasha Zhang, Wei Yang, Hao Chen, Bo Liu, Baixue Lin & Yong Tao (2019). Metabolic engineering for efficient supply of acetyl-CoA from different carbon sources in Escherichia coli. Microbial Cell Factories.