Escherichia coli JP204(pLS65)

Ancestors

Genotype with respect to wild type
supE44 hsdS20(rB−mB−) ara-14 proA2 lacY1 galK2 rpsL20 xyl-5 mtl-1 | Φ(pta-1::Tn10-lacZ) | ldhA::Kan | Plasmid pLS65 added

Bars (|) indicate differences between strains.


Plasmids

Production
Metabolites Heterologous Production type Production Biomass Carbon source Time Scale Ref.
(S)-lactate
Titer 45.0 g/L 7.2 g/L Glucose 67 h Fed-Batch [ 211 ]
acetate Substrate yield 0.04 mmol/mmol of sustrate Glucose Flask [ 211 ]
ethanol Substrate yield 0.24 mmol/mmol of sustrate Glucose Flask [ 211 ]
(S)-lactate
Substrate yield 0.72 mmol/mmol of sustrate Glucose Flask [ 211 ]
succinate Substrate yield 0.2 mmol/mmol of sustrate Glucose Flask [ 211 ]
pyruvate Substrate yield 0.0 mmol/mmol of sustrate Glucose Flask [ 211 ]
formate Substrate yield 0.21 mmol/mmol of sustrate Glucose Flask [ 211 ]
succinate Titer 5.4 g/L 7.2 g/L Glucose 67 h Fed-Batch [ 211 ]
(S)-lactate
Substrate yield 0.69 g/g of sustrate 7.2 g/L Glucose 67 h Fed-Batch [ 211 ]

* Inferred from plots using RetroPlot.


References