Escherichia coli W3110 trpD9923 PTS-
Ancestors
Derived strains
- Escherichia coli W3110 trpD9923PTS-/pv5Glk5GalP
- Escherichia coli W3110 trpD9923PTS-/pJLBaroGfbrtktA
- Escherichia coli W3110 trpD9923PTS-/pv5Glk5GalP/pJLBaroGfbrtktA
- Escherichia coli trpD9923 PTS-/pTrc-ant3
Genotype with respect to parental
ΔptsHI, crr::kmR
Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | trpD9923 | ΔptsHI, crr::kmRBars (|) indicate differences between strains.
Modifications
Gene | Organism | Modification |
---|---|---|
Silencing
|
||
Silencing
|
||
Silencing
|
Production
Metabolites | Heterologous | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
---|---|---|---|---|---|---|---|---|
anthranilate | Titer | 0.7 g/L | 1.04 g/L | Glucose | Flask | [ 151 ] | ||
anthranilate | Titer | 13.3 g/L | 23.3 g/L | Glucose | 74 h | Fed-Batch | [ 151 ] | |
acetate | Titer | 0.0 g/L | 23.3 g/L | Glucose | 74 h | Fed-Batch | [ 151 ] |
* Inferred from plots using RetroPlot.
References
- Víctor E Balderas-Hernández, Andrea Sabido-Ramos, Patricia Silva, Natividad Cabrera-Valladares, Georgina Hernández-Chávez, José L Báez-Viveros, Alfredo Martínez, Francisco Bolívar & Guillermo Gosset (2009). Metabolic engineering for improving anthranilate synthesis from glucose in Escherichia coli. Microbial Cell Factories.
- Balderas-Hernández, V.E., Treviño-Quintanilla, L.G., Hernández-Chávez, G. et al. Catechol biosynthesis from glucose in Escherichia coli anthranilate-overproducer strains by heterologous expression of anthranilate 1,2-dioxygenase from Pseudomonas aeruginosa PAO1. Microb Cell Fact 13, 136 (2014). https://doi.org/10.1186/s12934-014-0136-x