Escherichia coli XZ132

Ancestors

Genotype with respect to parental

Metabolic evolution in AM1 medium with 12% glucose


Genotype with respect to wild type
ΔpflB ΔfrdABCD ΔadhE ΔackA | ldhA::FRT-tet-FRT::alaDGs | Δtet | Metabolic evolution in NBS medium with 2% glucose | Metabolic evolution in NBS medium with 5% glucose | Metabolic evolution in NBS medium with 8% glucose | ΔmgsA | Metabolic evolution in NBS medium with 8% glucose | ΔdadX | Metabolic evolution in NBS medium with 8% glucose | Metabolic evolution in AM1 medium with 8% glucose | Metabolic evolution in AM1 medium with 10% glucose | Metabolic evolution in AM1 medium with 12% glucose

Bars (|) indicate differences between strains.


Strain generated through random mutagenesis


Production
Metabolites Heterologous Production type Production Biomass Carbon source Time Scale Ref.
L-alanine Titer 1279.0 mM 2.03 g/L Glucose 48 h Batch [ 208 ]
L-alanine Substrate yield 0.95 g/g of sustrate 2.03 g/L Glucose 48 h Batch [ 208 ]
succinate Titer 2.0 mM 2.03 g/L Glucose 48 h Batch [ 208 ]
acetate Titer 7.0 mM 2.03 g/L Glucose 48 h Batch [ 208 ]

* Inferred from plots using RetroPlot.


References