Escherichia coli LH15

Ancestors

All strains

Escherichia coli JM109

Escherichia coli SLH01

Escherichia coli SLH02

Escherichia coli SLH04

Escherichia coli SLH06

Escherichia coli SLH07

Escherichia coli SLH08

Escherichia coli SLH11

Escherichia coli LH15

Genotype with respect to wild type

recA1, thi, endA1, supE44, gyrA96, hsdR17 Δ(lac-proAB)/(lac-proAB)/F′[traD36,proAB+ , lacIq , lacZΔM15] | △tnaA::Km^R | △sdaA::Km^R | ΔyhaM::KanR | P_trc1-serA^fbrCB::Kan^R | P_cysM::P_trc1 | P_nrdH::P_trc1 | P_cysB::P_trc1 | Plasmid pLH03 added

Bars (|) indicate differences between strains.

Plasmids

pLH03 (P_cysE-cysE^fbr, P_GAPDH-ydeD)

Production

Metabolites	Heterologous	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
L-cysteine		Titer	155.26 mg/L	1.29 g/L^*	Glucose	48 h ^*	Flask	[ 227 ]
acetate		Titer	3.53 g/L ^*	1.29 g/L^*	Glucose	48 h ^*	Flask	[ 227 ]

* Inferred from plots using RetroPlot.

References

Han Liu, Yehua Hou, Yu Wang & Zhimin Li (2019). Enhancement of Sulfur Conversion Rate in the Production of l‑Cysteine by Engineered Escherichia coli. Journal of Agricultural & Food Chemistry.