Escherichia coli LH16
Ancestors
Genotype with respect to wild type
recA1, thi, endA1, supE44, gyrA96, hsdR17 Δ(lac-proAB)/(lac-proAB)/F′[traD36,proAB+ , lacIq , lacZΔM15] | △tnaA::KmR | △sdaA::KmR | ΔyhaM::KanR | Ptrc1-serAfbrCB::KanR | PcysM::Ptrc1 | PnrdH::Ptrc1 | Plasmid pTrc99a-cysB added | Plasmid pLH03 addedBars (|) indicate differences between strains.
Plasmids
-
pLH03 (PcysE-cysEfbr, PGAPDH-ydeD)
pTrc99a-cysB (cysB)
Production
Metabolites | Heterologous | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
---|---|---|---|---|---|---|---|---|
L-cysteine | Titer | 1359.12 mg/L | 1.58 g/L* | Glucose | 48 h * | Flask | [ 227 ] | |
L-cysteine | Titer | 7500.0 mg/L * | 8.64 g/L* | Glucose | 22 h * | Fed-Batch | [ 227 ] | |
acetate | Titer | 0.0 g/L * | 1.58 g/L* | Glucose | 48 h * | Flask | [ 227 ] | |
acetate | Titer | 0.28 g/L * | 8.64 g/L* | Glucose | 22 h * | Fed-Batch | [ 227 ] |
* Inferred from plots using RetroPlot.