Escherichia coli ita19

Ancestors

Derived strains

Genotype with respect to parental

∆pta


Genotype with respect to wild type
F+ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | Plasmid pCadCs added | ΔaceA | ∆sucCD | ∆pykA ∆pykF::cam | ∆pta

Bars (|) indicate differences between strains.


Plasmids

  • pCadCs (BBa_J23100_cadA_gltA_T, ColE1 origin, (T=Terminator (BBa_0010)))


Modifications
Gene Organism Modification
pta
Silencing

Production
Metabolites Heterologous Production type Production Biomass Carbon source Time Scale Ref.
itaconate
Titer 0.06 g/L Glucose Flask [ 234 ]
itaconate
Substrate yield 0.02 mol/mol of substrate Glucose Flask [ 234 ]
acetate Substrate yield 0.03 mol/mol of substrate Glucose Flask [ 234 ]

* Inferred from plots using RetroPlot.


References