Escherichia coli A6

Ancestors

All strains

Escherichia coli K12

Escherichia coli W1485

Escherichia coli MG1655

Escherichia coli A1

Escherichia coli A6

Derived strains

Escherichia coli A7

Genotype with respect to parental

ΔargR:: P_trc-argJ_Cg

Genotype with respect to wild type

F⁺ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | ΔargR:: P_trc-argJ_Cg

Bars (|) indicate differences between strains.

Modifications

Gene	Organism	Modification
argR	Escherichia coli	Silencing
argJ	Corynebacterium glutamicum	Overexpression

Production

Metabolites	Heterologous	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
L-arginine		Titer	12.8 g/L	17.5 OD₆₀₀^*	Glucose		Flask	[ 237 ]
acetate		Titer	12.4 g/L	17.5 OD₆₀₀^*	Glucose		Flask	[ 237 ]
L-arginine		Substrate yield	0.16 g/g of sustrate ^*	17.5 OD₆₀₀^*	Glucose		Flask	[ 237 ]
L-arginine		Titer	61.2 g/L	52.0 OD₆₀₀^*	Glucose		Fed-Batch	[ 237 ]
L-arginine		Substrate yield	0.289 g/g of sustrate	52.0 OD₆₀₀^*	Glucose		Fed-Batch	[ 237 ]
acetate		Titer	12.43 g/L ^*	52.0 OD₆₀₀^*	Glucose		Fed-Batch	[ 237 ]

* Inferred from plots using RetroPlot.

References

Wang, HD., Xu, JZ. & Zhang, WG. Metabolic engineering of Escherichia coli for efficient production of l-arginine. Appl Microbiol Biotechnol 106, 5603–5613 (2022).