Escherichia coli A7

Ancestors

All strains

Escherichia coli K12

Escherichia coli W1485

Escherichia coli MG1655

Escherichia coli A1

Escherichia coli A6

Escherichia coli A7

Genotype with respect to parental

P_trc-argC-J-B-D-F

Genotype with respect to wild type

F⁺ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | ΔargR:: P_trc-argJ_Cg | P_trc-argC-J-B-D-F

Bars (|) indicate differences between strains.

Modifications

Gene	Organism	Modification
argC	Corynebacterium glutamicum	Overexpression
argJ	Corynebacterium glutamicum	Overexpression
argB	Corynebacterium glutamicum	Overexpression
argD	Corynebacterium glutamicum	Overexpression
argF	Corynebacterium glutamicum	Overexpression

Production

Metabolites	Heterologous	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
L-arginine		Titer	70.1 g/L	50.29 OD₆₀₀^*	Glucose	60 h ^*	Fed-Batch	[ 237 ]
L-arginine		Substrate yield	0.326 g/g of sustrate	50.29 OD₆₀₀^*	Glucose	60 h ^*	Fed-Batch	[ 237 ]
L-arginine		Volumetric productivity	1.17 g/L/h	50.29 OD₆₀₀^*	Glucose	60 h ^*	Fed-Batch	[ 237 ]
L-arginine		Titer	16.9 g/L	16.9 OD₆₀₀^*	Glucose		Flask	[ 237 ]
acetate		Titer	1.8 g/L	16.9 OD₆₀₀^*	Glucose		Flask	[ 237 ]
acetate		Titer	15.7 g/L	50.29 OD₆₀₀^*	Glucose	60 h ^*	Fed-Batch	[ 237 ]
L-arginine		Substrate yield	0.209 g/g of sustrate ^*	16.9 OD₆₀₀^*	Glucose		Flask	[ 237 ]

* Inferred from plots using RetroPlot.

References

Wang, HD., Xu, JZ. & Zhang, WG. Metabolic engineering of Escherichia coli for efficient production of l-arginine. Appl Microbiol Biotechnol 106, 5603–5613 (2022).