Tong Shen, Qing Liu, Xixian Xie, Qingyang Xu & Ning Chen (2012). Improved Production of Tryptophan in Genetically Engineered Escherichia coli with TktA and PpsA Overexpression. BioMed Research International.

Strains

• Escherichia coli TRTH1013
• Escherichia coli TRTH0709
• Escherichia coli TRTH1011
• Escherichia coli TRTH1012

Plasmids

• pBR322
• pSV-709
• pMEL01
• pSTV28
• pMEL02
• pMEL03

Metabolite production

• L-tryptophan
  Substrate yield
  • 0.125 mol/mol of substrate (Escherichia coli TRTH1011 in Fed-Batch)
  • 0.145 mol/mol of substrate (Escherichia coli TRTH1013 in Fed-Batch)
  • 0.13 mol/mol of substrate (Escherichia coli TRTH0709 in Fed-Batch)
  • 0.132 mol/mol of substrate (Escherichia coli TRTH1012 in Fed-Batch)
  Titer
  • 36.3 g/L (Escherichia coli TRTH1011 in Fed-Batch)
  • 40.2 g/L (Escherichia coli TRTH1013 in Fed-Batch)
  • 35.9 g/L (Escherichia coli TRTH0709 in Fed-Batch)
  • 37.9 g/L (Escherichia coli TRTH1012 in Fed-Batch)