Zhu Li, Dongqin Ding, Huiying Wang, Linxia Liu, Huan Fang, Tao Chen & Dawei Zhang (2020). Engineering Escherichia coli to improve tryptophan production via genetic manipulation of precursor and cofactor pathways. Synthetic and Systems Biotechnology.

Strains

• Escherichia coli KW001
• Escherichia coli TS-1
• Escherichia coli TS-2
• Escherichia coli TS-3
• Escherichia coli TS-4
• Escherichia coli TS-5
• Escherichia coli TS-51
• Escherichia coli TS-52
• Escherichia coli TS-6
• Escherichia coli TS-7
• Escherichia coli TS-8
• Escherichia coli TS-9

Plasmids

• pXlltS

Metabolite production

• acetate
  Titer
  • 0.798 g/L (Escherichia coli TS-1 in Flask)
  • 0.185 g/L (Escherichia coli TS-5 in Flask)
  • 0.34 g/L (Escherichia coli TS-51 in Flask)
  • 0.076 g/L (Escherichia coli TS-10 in Flask)
  • 0.835 g/L (Escherichia coli KW001 in Flask)
  • 0.786 g/L (Escherichia coli TS-52 in Flask)
  • 0.138 g/L (Escherichia coli TS-6 in Flask)
  • 0.276 g/L (Escherichia coli TS-7 in Flask)
  • 0.138 g/L (Escherichia coli TS-8 in Flask)
  • 0.124 g/L (Escherichia coli TS-9 in Flask)
• L-glutamine
  Titer
  • 0.0 g/L (Escherichia coli TS-1 in Flask)
  • 0.0 g/L (Escherichia coli KW001 in Flask)
• 2-oxoglutarate
  Titer
  • 1.492 g/L (Escherichia coli TS-5 in Flask)
  • 0.261 g/L (Escherichia coli KW001 in Flask)
  • 0.362 g/L (Escherichia coli TS-1 in Flask)
  • 0.671 g/L (Escherichia coli TS-51 in Flask)
  • 0.281 g/L (Escherichia coli TS-52 in Flask)
  • 1.242 g/L (Escherichia coli TS-6 in Flask)
  • 1.09 g/L (Escherichia coli TS-8 in Flask)
  • 1.248 g/L (Escherichia coli TS-9 in Flask)
  • 1.104 g/L (Escherichia coli TS-10 in Flask)
  • 1.241 g/L (Escherichia coli TS-7 in Flask)
• L-serine
  Titer
  • 0.05 g/L (Escherichia coli TS-8 in Flask)
  • 0.0 g/L (Escherichia coli TS-6 in Flask)
• L-tryptophan
  Titer
  • 0.62 g/L (Escherichia coli KW001 in Flask)
  • 0.81 g/L (Escherichia coli TS-1 in Flask)
  • 1.06 g/L (Escherichia coli TS-5 in Flask)
  • 1.22 g/L (Escherichia coli TS-6 in Flask)
  • 1.04 g/L (Escherichia coli TS-7 in Flask)
  • 1.38 g/L (Escherichia coli TS-8 in Flask)
  • 1.52 g/L (Escherichia coli TS-9 in Flask)
  • 1.71 g/L (Escherichia coli TS-10 in Flask)
  • 0.76 g/L (Escherichia coli TS-51 in Flask)
  • 0.75 g/L (Escherichia coli TS-52 in Flask)
  • 0.62 g/L (Escherichia coli TS-2 in Flask)
  Substrate yield
  • 4.1 % (Escherichia coli TS-5 in Flask)
  • 5.4 % (Escherichia coli TS-8 in Flask)
  • 6.7 % (Escherichia coli TS-10 in Flask)