Huimin Liu, Junhua Kang, Qingsheng Qi & Guanjun Chen (2010). Production of Lactate in Escherichia coli by Redox Regulation Genetically and Physiologically. Applied Biochemistry and Biotechnology.

Strains

• Escherichia coli SDU4
• Escherichia coli SDU2
• Escherichia coli W3110

Metabolite production

• succinate
  Substrate yield
  • 0.09 mol/mol of substrate (Escherichia coli W3110 in Batch)
  • 0.08 mol/mol of substrate (Escherichia coli SDU2 in Batch)
  • 0.07 mol/mol of substrate (Escherichia coli SDU4 in Batch)
  Titer
  • 1.16 g/L (Escherichia coli SDU4 in Batch)
  • 9.79 mM (Escherichia coli SDU4 in Batch)
  • 5.55 mM (Escherichia coli SDU4 in Batch)
  • 0.66 g/L (Escherichia coli SDU4 in Batch)
  • 0.21 g/L (Escherichia coli SDU4 in Batch)
  • 1.79 mM (Escherichia coli SDU4 in Batch)
  • 3.88 mM (Escherichia coli SDU4 in Batch)
  • 0.46 g/L (Escherichia coli SDU4 in Batch)
  • 0.0 g/L (Escherichia coli SDU4 in Batch)
  • 10.16 mM (Escherichia coli SDU4 in Batch)
  • 1.2 g/L (Escherichia coli SDU4 in Batch)
  • 11.05 mM (Escherichia coli SDU4 in Batch)
  • 1.33 g/L (Escherichia coli SDU4 in Batch)
  • 11.22 mM (Escherichia coli SDU4 in Batch)
  • 0.97 g/L (Escherichia coli SDU4 in Batch)
  • 8.19 mM (Escherichia coli SDU4 in Batch)
  • 1.3 g/L (Escherichia coli SDU4 in Batch)
• formate
  Substrate yield
  • 0.43 mol/mol of substrate (Escherichia coli W3110 in Batch)
  • 0.03 mol/mol of substrate (Escherichia coli SDU2 in Batch)
  • 0.0 mol/mol of substrate (Escherichia coli SDU4 in Batch)
• acetate
  Substrate yield
  • 0.4 mol/mol of substrate (Escherichia coli W3110 in Batch)
  • 0.02 mol/mol of substrate (Escherichia coli SDU2 in Batch)
  • 0.18 mol/mol of substrate (Escherichia coli SDU4 in Batch)
• ethanol
  Substrate yield
  • 0.18 mol/mol of substrate (Escherichia coli W3110 in Batch)
  • 0.09 mol/mol of substrate (Escherichia coli SDU2 in Batch)
  • 0.0 mol/mol of substrate (Escherichia coli SDU4 in Batch)
• (R)-lactate
  Titer
  • 4.42 g/L (Escherichia coli SDU4 in Batch)
  • 100.0 g/L (Escherichia coli SDU4 in Batch)
  • 179.15 mM (Escherichia coli SDU4 in Batch)
  • 16.14 g/L (Escherichia coli SDU4 in Batch)
  • 225.01 mM (Escherichia coli SDU4 in Batch)
  • 20.27 g/L (Escherichia coli SDU4 in Batch)
  • 24.11 g/L (Escherichia coli SDU4 in Batch)
  • 267.66 mM (Escherichia coli SDU4 in Batch)
  • 182.21 mM (Escherichia coli SDU4 in Batch)
  • 16.41 g/L (Escherichia coli SDU4 in Batch)
  • 49.07 mM (Escherichia coli SDU4 in Batch)
  • 18.59 g/L (Escherichia coli SDU4 in Batch)
  • 206.36 mM (Escherichia coli SDU4 in Batch)
  • 19.04 g/L (Escherichia coli SDU4 in Batch)
  • 211.42 mM (Escherichia coli SDU4 in Batch)
  • 18.77 g/L (Escherichia coli SDU4 in Batch)
  • 208.42 mM (Escherichia coli SDU4 in Batch)
  • 17.81 g/L (Escherichia coli SDU4 in Batch)
  • 197.74 mM (Escherichia coli SDU4 in Batch)
  Substrate yield
  • 1.34 mol/mol of substrate (Escherichia coli W3110 in Batch)
  • 1.67 mol/mol of substrate (Escherichia coli SDU2 in Batch)
  • 1.97 mol/mol of substrate (Escherichia coli SDU4 in Batch)
  • 1.73 mol/mol of substrate (Escherichia coli SDU4 in Batch)