Pengfei Gu, Fan Yang, Tianyuan Su, Fangfang Li, Yikui Li & Qingsheng Qi (2014). Construction of an l-serine producing Escherichia coli via metabolic engineering. Journal of Industrial Microbiology & Biotechnology.

Strains

• Escherichia coli YF-8
• Escherichia coli YF-6
• Escherichia coli YF-5
• Escherichia coli YF-4
• Escherichia coli YF-3
• Escherichia coli YF-7
• Escherichia coli LMJ-1
• Escherichia coli QZ1111
• Escherichia coli DH5α

Plasmids

• pBBR1MCS-2
• pKJ-1
• pYF-1
• pYF-2

Metabolite production

• pyruvate
  Titer
  • 1.95 g/L (Escherichia coli YF-3 in Flask)
  • 2.23 g/L (Escherichia coli DH5α in Flask)
  • 0.08 g/L (Escherichia coli YF-7 in Flask)
  • 0.38 g/L (Escherichia coli YF-8 in Flask)
• acetate
  Titer
  • 1.73 g/L (Escherichia coli YF-7 in Flask)
  • 2.02 g/L (Escherichia coli YF-8 in Flask)
• succinate
  Titer
  • 0.53 g/L (Escherichia coli YF-7 in Flask)
  • 0.86 g/L (Escherichia coli YF-8 in Flask)
• (R)-2-hydroxyglutarate
  Titer
  • 0.4 g/L (Escherichia coli YF-3 in Flask)
  • 0.28 g/L (Escherichia coli YF-4 in Flask)
  • 0.15 g/L (Escherichia coli YF-5 in Flask)
  • 0.35 g/L (Escherichia coli YF-6 in Flask)
  • 1.92 g/L (Escherichia coli YF-7 in Flask)
  • 1.54 g/L (Escherichia coli YF-8 in Flask)
• L-serine
  Titer
  • 1.35 g/L (Escherichia coli DH5α in Flask)
  • 1.52 g/L (Escherichia coli YF-3 in Flask)
  • 2.03 g/L (Escherichia coli YF-4 in Flask)
  • 2.29 g/L (Escherichia coli YF-5 in Flask)
  • 2.51 g/L (Escherichia coli YF-6 in Flask)
  • 4.5 g/L (Escherichia coli YF-7 in Flask)
  • 8.34 g/L (Escherichia coli YF-7 in Fed-Batch)
  • 3.7 g/L (Escherichia coli YF-8 in Flask)
• (S)-lactate
  Titer
  • 1.09 g/L (Escherichia coli YF-7 in Flask)
  • 1.38 g/L (Escherichia coli YF-8 in Flask)