Lina Liu, Xuguo Duan & Jing Wu (2016). L-Tryptophan Production in Escherichia coli Improved by Weakening the Pta-AckA Pathway. PLOS ONE.

Strains

• Escherichia coli FB-04
• Escherichia coli FB-04(pta1)
• Escherichia coli FB-04(ΔackA)
• Escherichia coli FB-04(Δpta)

Plasmids

• pSV03

Metabolite production

• acetate
  Titer
  • 8.4 g/L (Escherichia coli FB-04(pta1) in Flask)
  • 3.6 g/L (Escherichia coli FB-04(Δpta) in Flask)
  • 4.9 g/L (Escherichia coli FB-04(ΔackA) in Flask)
  • 0.5 g/L (Escherichia coli FB-04(Δpta) in Fed-Batch)
  • 1.5 g/L (Escherichia coli FB-04(ΔackA) in Fed-Batch)
  • 2.1 g/L (Escherichia coli FB-04(pta1) in Fed-Batch)
  • 12.9 g/L (Escherichia coli FB-04/pSV03 in Flask)
  • 4.3 g/L (Escherichia coli FB-04/pSV03 in Fed-Batch)
• L-tryptophan
  Titer
  • 1.4 g/L (Escherichia coli FB-04(Δpta) in Flask)
  • 21.8 g/L (Escherichia coli FB-04(Δpta) in Fed-Batch)
  • 1.3 g/L (Escherichia coli FB-04(ΔackA) in Flask)
  • 40.2 g/L (Escherichia coli FB-04(ΔackA) in Fed-Batch)
  • 44.0 g/L (Escherichia coli FB-04(pta1) in Fed-Batch)
  • 2.09 g/L (Escherichia coli FB-04(pta1) in Flask)
  • 1.1 g/L (Escherichia coli FB-04/pSV03 in Flask)
  • 38.1 g/L (Escherichia coli FB-04/pSV03 in Fed-Batch)
  Substrate yield
  • 0.04 g/g of sustrate (Escherichia coli FB-04(Δpta) in Flask)
  • 0.08 g/g of sustrate (Escherichia coli FB-04(Δpta) in Fed-Batch)
  • 0.032 g/g of sustrate (Escherichia coli FB-04(ΔackA) in Flask)
  • 0.12 g/g of sustrate (Escherichia coli FB-04(ΔackA) in Fed-Batch)
  • 0.049 g/g of sustrate (Escherichia coli FB-04(pta1) in Flask)
  • 0.13 g/g of sustrate (Escherichia coli FB-04(pta1) in Fed-Batch)
  • 0.02 g/g of sustrate (Escherichia coli FB-04/pSV03 in Flask)
  • 0.11 g/g of sustrate (Escherichia coli FB-04/pSV03 in Fed-Batch)
  Volumetric productivity
  • 0.029 g/L/h (Escherichia coli FB-04(Δpta) in Flask)
  • 0.4 g/L/h (Escherichia coli FB-04(Δpta) in Fed-Batch)
  • 0.74 g/L/h (Escherichia coli FB-04(ΔackA) in Flask)
  • 0.74 g/L/h (Escherichia coli FB-04(ΔackA) in Fed-Batch)
  • 0.044 g/L/h (Escherichia coli FB-04(pta1) in Flask)
  • 0.82 g/L/h (Escherichia coli FB-04(pta1) in Fed-Batch)
  • 0.02 g/L/h (Escherichia coli FB-04/pSV03 in Flask)
  • 0.7 g/L/h (Escherichia coli FB-04/pSV03 in Fed-Batch)