Víctor E Balderas-Hernández, Andrea Sabido-Ramos, Patricia Silva, Natividad Cabrera-Valladares, Georgina Hernández-Chávez, José L Báez-Viveros, Alfredo Martínez, Francisco Bolívar & Guillermo Gosset (2009). Metabolic engineering for improving anthranilate synthesis from glucose in Escherichia coli. Microbial Cell Factories.

Strains

• Escherichia coli W3110 trpD9923/pJLBaroG^fbr
• Escherichia coli W3110 trpD9923PTS^-/pv5Glk5GalP
• Escherichia coli W3110 trpD9923PTS^-/pJLBaroG^fbrtktA
• Escherichia coli W3110 trpD9923PTS-/pv5Glk5GalP/pJLBaroG^fbrtktA
• Escherichia coli W3110 trpD9923/pJLBaroG^fbrtktA
• Escherichia coli W3110 trpD9923
• Escherichia coli W3110 trpD9923 PTS^-

Plasmids

• pJLBaroG^fbr

Metabolite production

• acetate
  Titer
  • 3.18 g/L (Escherichia coli W3110 trpD9923/pJLBaroG^fbr in Fed-Batch)
  • 0.0 g/L (Escherichia coli W3110 trpD9923 PTS^- in Fed-Batch)
  • 0.0 g/L (Escherichia coli W3110 trpD9923PTS^-/pv5Glk5GalP in Fed-Batch)
  • 0.0 g/L (Escherichia coli W3110 trpD9923PTS^-/pJLBaroG^fbrtktA in Fed-Batch)
  • 0.0 g/L (Escherichia coli W3110 trpD9923PTS-/pv5Glk5GalP/pJLBaroG^fbrtktA in Fed-Batch)
  • 0.5 g/L (Escherichia coli W3110 trpD9923/pJLBaroG^fbrtktA in Fed-Batch)
  • 9.65 g/L (Escherichia coli W3110 trpD9923 in Fed-Batch)
• anthranilate
  Titer
  • 0.31 g/L (Escherichia coli W3110 trpD9923 in Flask)
  • 4.21 g/L (Escherichia coli W3110 trpD9923 in Fed-Batch)
  • 0.7 g/L (Escherichia coli W3110 trpD9923 PTS^- in Flask)
  • 13.3 g/L (Escherichia coli W3110 trpD9923 PTS^- in Fed-Batch)
  • 0.33 g/L (Escherichia coli W3110 trpD9923PTS^-/pv5Glk5GalP in Flask)
  • 5.3 g/L (Escherichia coli W3110 trpD9923PTS^-/pv5Glk5GalP in Fed-Batch)
  • 0.45 g/L (Escherichia coli W3110 trpD9923PTS^-/pJLBaroG^fbrtktA in Flask)
  • 7.16 g/L (Escherichia coli W3110 trpD9923PTS^-/pJLBaroG^fbrtktA in Fed-Batch)
  • 0.33 g/L (Escherichia coli W3110 trpD9923PTS-/pv5Glk5GalP/pJLBaroG^fbrtktA in Flask)
  • 5.08 g/L (Escherichia coli W3110 trpD9923PTS-/pv5Glk5GalP/pJLBaroG^fbrtktA in Fed-Batch)
  • 14.0 g/L (Escherichia coli W3110 trpD9923/pJLBaroG^fbrtktA in Fed-Batch)
  • 0.75 g/L (Escherichia coli W3110 trpD9923/pJLBaroG^fbrtktA in Flask)
  • 0.44 g/L (Escherichia coli W3110 trpD9923/pJLBaroG^fbr in Flask)
  • 6.72 g/L (Escherichia coli W3110 trpD9923/pJLBaroG^fbr in Fed-Batch)
  Substrate yield
  • 0.2 g/g of sustrate (Escherichia coli W3110 trpD9923/pJLBaroG^fbrtktA in Fed-Batch)