Pengfei Gu, Fan Yang, Fangfang Li, Quanfeng Liang & Qingsheng Qi (2013). Knocking out analysis of tryptophan permeases in Escherichia coli for improving L-tryptophan production. Applied Microbiology and Biotechnology.

Strains

• Escherichia coli GPT201
• Escherichia coli GPT202
• Escherichia coli GPT204
• Escherichia coli GPT205
• Escherichia coli GPT206
• Escherichia coli GPT207
• Escherichia coli GPT1011
• Escherichia coli GPT1012
• Escherichia coli GPT203
• Escherichia coli GPT1013
• Escherichia coli GPT1014
• Escherichia coli GPT1015
• Escherichia coli GPT1016
• Escherichia coli GPT1017
• Escherichia coli GPT1002
• Escherichia coli GPT101

Plasmids

• pTAT

Metabolite production

• acetate
  Titer
  • 1.05 g/L (Escherichia coli GPT1002 in Batch)
  • 1.12 g/L (Escherichia coli GPT1011 in Batch)
  • 1.08 g/L (Escherichia coli GPT1012 in Batch)
  • 0.96 g/L (Escherichia coli GPT1013 in Batch)
  • 1.22 g/L (Escherichia coli GPT1014 in Batch)
  • 8.12 g/L (Escherichia coli GPT1015 in Batch)
  • 8.44 g/L (Escherichia coli GPT1016 in Batch)
  • 1.02 g/L (Escherichia coli GPT1017 in Batch)
• L-tryptophan
  Titer
  • 1.84 g/L (Escherichia coli GPT1002 in Batch)
  • 2.79 g/L (Escherichia coli GPT1017 in Batch)
  • 16.3 g/L (Escherichia coli GPT1017 in Fed-Batch)
  • 2.44 g/L (Escherichia coli GPT1014 in Batch)
  • 0.06 g/L (Escherichia coli GPT1015 in Batch)
  • 1.81 g/L (Escherichia coli GPT1011 in Batch)
  • 0.07 g/L (Escherichia coli GPT1016 in Batch)
  • 2.05 g/L (Escherichia coli GPT1012 in Batch)
  • 1.75 g/L (Escherichia coli GPT1013 in Batch)