T. B. Causey, K. T. Shanmugam, L. P. Yomano & L. O. Ingram (2004). Engineering Escherichia coli for efficient conversion of glucose to pyruvate. Proceedings of the National Academy of Sciences of the United States of America.

Strains

• Escherichia coli LY74
• Escherichia coli SZ61
• Escherichia coli TC37
• Escherichia coli TC38
• Escherichia coli TC41
• Escherichia coli TC42
• Escherichia coli TC43
• Escherichia coli TC44
• Escherichia coli TC36
• Escherichia coli W3110

Plasmids

• pCR2.1-TOPO

Metabolite production

• acetate
  Titer
  • 180.0 mM (Escherichia coli W3110 in Batch)
  • 178.4 mM (Escherichia coli TC42 in Batch)
  • 62.4 mM (Escherichia coli TC44 in Fed-Batch)
  • 7.0 mM (Escherichia coli TC38 in Batch)
  • 197.7 mM (Escherichia coli TC36 in Batch)
  • 11.6 mM (Escherichia coli TC44 in Batch)
• 2-oxoglutarate
  Titer
  • 8.3 mM (Escherichia coli W3110 in Batch)
  • 76.2 mM (Escherichia coli TC42 in Batch)
  • 45.3 mM (Escherichia coli TC44 in Fed-Batch)
  • 7.9 mM (Escherichia coli TC38 in Batch)
  • 16.6 mM (Escherichia coli TC36 in Batch)
  • 3.6 mM (Escherichia coli TC44 in Batch)
• succinate
  Titer
  • 13.7 mM (Escherichia coli W3110 in Batch)
  • 24.3 mM (Escherichia coli TC42 in Batch)
  • 14.7 mM (Escherichia coli TC44 in Fed-Batch)
  • 2.0 mM (Escherichia coli TC38 in Batch)
  • 13.7 mM (Escherichia coli TC36 in Batch)
  • 16.8 mM (Escherichia coli TC44 in Batch)
• fumarate
  Titer
  • 0.9 mM (Escherichia coli W3110 in Batch)
  • 1.7 mM (Escherichia coli TC42 in Batch)
  • 3.3 mM (Escherichia coli TC44 in Fed-Batch)
  • 0.2 mM (Escherichia coli TC38 in Batch)
  • 1.4 mM (Escherichia coli TC36 in Batch)
  • 1.1 mM (Escherichia coli TC44 in Batch)
• pyruvate
  Titer
  • 20.8 mM (Escherichia coli W3110 in Batch)
  • 81.0 mM (Escherichia coli TC36 in Fed-Batch)
  • 38.1 mM (Escherichia coli TC36 in Batch)
  • 241.9 mM (Escherichia coli TC38 in Batch)
  • 79.0 mM (Escherichia coli TC42 in Batch)
  • 252.5 mM (Escherichia coli TC44 in Batch)
  • 749.0 mM (Escherichia coli TC44 in Fed-Batch)