Sang Jun Lee, Dong-Yup Lee, Tae Yong Kim, Byung Hun Kim, Jinwon Lee & Sang Yup Lee (2005). Metabolic Engineering of Escherichia coli for Enhanced Production of Succinic Acid, Based on Genome Comparison and In Silico Gene Knockout Simulation. Applied and Environmental Microbiology.

Strains

• Escherichia coli W3110G
• Escherichia coli W3110GF
• Escherichia coli W3110GFO
• Escherichia coli W3110GFH
• Escherichia coli W3110GFHO
• Escherichia coli W3110GFHOE
• Escherichia coli W3110GFA
• Escherichia coli W3110GFAP
• Escherichia coli W3110GFAPL
• Escherichia coli MG-104
• Escherichia coli W3110

Metabolite production

• (R)-lactate
  Titer
  • 10.62 mM (Escherichia coli W3110 in Flask)
  • 13.71 mM (Escherichia coli W3110G in Flask)
  • 14.13 mM (Escherichia coli W3110GF in Flask)
  • 12.97 mM (Escherichia coli W3110GFO in Flask)
  • 15.18 mM (Escherichia coli W3110GFH in Flask)
  • 17.06 mM (Escherichia coli W3110GFHO in Flask)
  • 13.31 mM (Escherichia coli W3110GFHOE in Flask)
  • 5.47 mM (Escherichia coli W3110GFA in Flask)
  • 10.67 mM (Escherichia coli W3110GFA in Flask)
  • 21.4 mM (Escherichia coli W3110GFAP in Flask)
  • 0.0 mM (Escherichia coli W3110GFAPL in Flask)
• formate
  Titer
  • 88.03 mM (Escherichia coli W3110 in Flask)
  • 88.57 mM (Escherichia coli W3110G in Flask)
  • 86.25 mM (Escherichia coli W3110GF in Flask)
  • 88.39 mM (Escherichia coli W3110GFO in Flask)
  • 85.86 mM (Escherichia coli W3110GFH in Flask)
  • 90.4 mM (Escherichia coli W3110GFHO in Flask)
  • 85.98 mM (Escherichia coli W3110GFHOE in Flask)
  • 27.47 mM (Escherichia coli W3110GFA in Flask)
  • 50.36 mM (Escherichia coli W3110GFA in Flask)
  • 0.0 mM (Escherichia coli W3110GFAP in Flask)
  • 0.0 mM (Escherichia coli W3110GFAPL in Flask)
• acetate
  Titer
  • 40.1 mM (Escherichia coli W3110 in Flask)
  • 40.69 mM (Escherichia coli W3110G in Flask)
  • 40.1 mM (Escherichia coli W3110GF in Flask)
  • 40.89 mM (Escherichia coli W3110GFO in Flask)
  • 38.88 mM (Escherichia coli W3110GFH in Flask)
  • 40.4 mM (Escherichia coli W3110GFHO in Flask)
  • 38.66 mM (Escherichia coli W3110GFHOE in Flask)
  • 16.48 mM (Escherichia coli W3110GFA in Flask)
  • 30.25 mM (Escherichia coli W3110GFA in Flask)
  • 4.1 mM (Escherichia coli W3110GFAP in Flask)
  • 0.0 mM (Escherichia coli W3110GFAPL in Flask)
• ethanol
  Titer
  • 5.77 mM (Escherichia coli W3110 in Flask)
  • 5.98 mM (Escherichia coli W3110G in Flask)
  • 5.92 mM (Escherichia coli W3110GF in Flask)
  • 6.0 mM (Escherichia coli W3110GFO in Flask)
  • 6.2 mM (Escherichia coli W3110GFHO in Flask)
  • 5.84 mM (Escherichia coli W3110GFHOE in Flask)
  • 1.88 mM (Escherichia coli W3110GFA in Flask)
  • 4.14 mM (Escherichia coli W3110GFA in Flask)
  • 5.5 mM (Escherichia coli W3110GFAP in Flask)
  • 1.6 mM (Escherichia coli W3110GFAPL in Flask)
• succinate
  Titer
  • 2.43 mM (Escherichia coli W3110 in Flask)
  • 2.16 mM (Escherichia coli W3110G in Flask)
  • 2.83 mM (Escherichia coli W3110GF in Flask)
  • 2.67 mM (Escherichia coli W3110GFO in Flask)
  • 2.51 mM (Escherichia coli W3110GFH in Flask)
  • 2.58 mM (Escherichia coli W3110GFHO in Flask)
  • 2.49 mM (Escherichia coli W3110GFHOE in Flask)
  • 8.16 mM (Escherichia coli W3110GFA in Flask)
  • 17.35 mM (Escherichia coli W3110GFA in Flask)
  • 8.45 mM (Escherichia coli W3110GFAP in Flask)
  • 0.39 mM (Escherichia coli W3110GFAPL in Flask)