Jun Hyoung Lee, Bong Hyun Sung, Mi Sun Kim, Frederick R Blattner, Byoung Hoon Yoon, Jung Hoe Kim & Sun Chang Kim (2009). Metabolic engineering of a reduced-genome strain of Escherichia coli for L-threonine production. Microbial Cell Factories.
Strains
- Escherichia coli MDS42
- Escherichia coli MG-102
- Escherichia coli MG-103
- Escherichia coli MG-105
- Escherichia coli MDS-202
- Escherichia coli MDS-203
- Escherichia coli MDS-204
- Escherichia coli MDS-205
- Escherichia coli MG1655 thrB::Tn5
- Escherichia coli N99 rhtA23 thr::Tn10
- Escherichia coli ATCC 21277
- Escherichia coli MG1655
- Escherichia coli MG-104
Metabolite production
-
L-threonine
-
Titer
- 16.55 mg/L (Escherichia coli MG1655 in Flask)
- 19.61 mg/L (Escherichia coli MDS42 in Flask)
- 36.61 mg/L (Escherichia coli MDS-202 in Flask)
- 79.94 mg/L (Escherichia coli MDS-203 in Flask)
- 228.43 mg/L (Escherichia coli MDS-204 in Flask)
- 303.23 mg/L (Escherichia coli MDS-205 in Flask)
- 40.1 g/L (Escherichia coli MDS-205 in Batch)
- 34.06 mg/L (Escherichia coli MG-102 in Flask)
- 42.25 mg/L (Escherichia coli MG-103 in Flask)
- 138.01 mg/L (Escherichia coli MG-104 in Flask)
- 165.54 mg/L (Escherichia coli MG-105 in Flask) Substrate yield
- 0.401 g/g of sustrate (Escherichia coli MDS-205 in Batch)