N. Yakandawala, T. Romeo, A. D. Friesen & S. Madhyastha (2007). Metabolic engineering of Escherichia coli to enhance phenylalanine production. Applied Microbiology and Biotechnology.

Strains

• Escherichia coli TRMG1655
• Escherichia coli DCMG1655
• Escherichia coli NST37
• Escherichia coli NSTCSRA
• Escherichia coli NSTCSRD
• Escherichia coli NSTCSRACSRD
• Escherichia coli NST [pCsrB]
• Escherichia coli NST [pTktA]
• Escherichia coli NSTCSRA [pCsrB]
• Escherichia coli NST [pZwf]
• Escherichia coli NSTCSRA [pTktA]
• Escherichia coli NSTCSRA [pZwf]
• Escherichia coli NSTCSRA [pTkaFpA]

Plasmids

• pQE60
• pCsrB
• pZwf
• pTktA
• pTkaFpA

Metabolite production

• L-phenylalanine
  Titer
  • 1194.87 mg/L (Escherichia coli NST37 in Flask)
  • 2391.5 mg/L (Escherichia coli NSTCSRA [pTktA] in Flask)
  • 2334.73 mg/L (Escherichia coli NST [pCsrB] in Flask)
  • 1840.0 mg/L (Escherichia coli NSTCSRA [pTkaFpA] in Flask)
  • 1669.84 mg/L (Escherichia coli NSTCSRA in Flask)
  • 1612.07 mg/L (Escherichia coli NSTCSRD in Flask)
  • 1606.0 mg/L (Escherichia coli NSTCSRA [pZwf] in Flask)
  • 1521.57 mg/L (Escherichia coli NSTCSRA [pCsrB] in Flask)
  • 1415.42 mg/L (Escherichia coli NSTCSRACSRD in Flask)
  • 1265.75 mg/L (Escherichia coli NST [pZwf] in Flask)
  • 1217.11 mg/L (Escherichia coli NST [pTktA] in Flask)
  Biomass-specific productivity
  • 594.86 mg/L/OD₆₀₀ (Escherichia coli NST37 in Flask)
  • 864.54 mg/L/OD₆₀₀ (Escherichia coli NSTCSRACSRD in Flask)
  • 1030.14 mg/L/OD₆₀₀ (Escherichia coli NSTCSRA [pCsrB] in Flask)
  • 1282.33 mg/L/OD₆₀₀ (Escherichia coli NSTCSRA [pTktA] in Flask)
  • 938.0 mg/L/OD₆₀₀ (Escherichia coli NSTCSRA [pZwf] in Flask)
  • 1021.66 mg/L/OD₆₀₀ (Escherichia coli NSTCSRA [pTkaFpA] in Flask)
  • 910.95 mg/L/OD₆₀₀ (Escherichia coli NSTCSRD in Flask)
  • 1633.52 mg/L/OD₆₀₀ (Escherichia coli NST [pCsrB] in Flask)
  • 719.06 mg/L/OD₆₀₀ (Escherichia coli NST [pTktA] in Flask)
  • 737.66 mg/L/OD₆₀₀ (Escherichia coli NST [pZwf] in Flask)
  • 1009.0 mg/L/OD₆₀₀ (Escherichia coli NSTCSRA in Flask)