Jian‐Feng Huang, Zhi‐Qiang Liu, Li‐Qun Jin, Xiao‐Ling Tang, Zhen‐Yang Shen, Huan‐Huan Yin & Yu‐Guo Zheng (2016). Metabolic engineering of Escherichia coli for microbial production of L‐methionine. Biotechnology & Bioengineering.

Strains

Plasmids

Metabolite production

L-aspartate
- 1.47 g/L (Escherichia coli W3110 ΔmetJΔmetIΔlysA/pTrcA*H in Fed-Batch)
L-glutamate
- 1.55 g/L (Escherichia coli W3110 ΔmetJΔmetIΔlysA/pTrcA*H in Fed-Batch)
acetate
- 1.78 g/L (Escherichia coli W3110 ΔmetJΔmetIΔlysA/pTrcA*H in Fed-Batch)
- 28.9 mM (Escherichia coli TC38 in Batch)
2-oxoglutarate
- 10.5 mM (Escherichia coli TC38 in Batch)
succinate
- 8.1 mM (Escherichia coli TC38 in Batch)
fumarate
- 0.8 mM (Escherichia coli TC38 in Batch)
L-methionine
- 0.0 g/L (Escherichia coli W3110 in Flask)
- 4.09 g/L (Escherichia coli W3110 ΔmetJΔmetIΔlysAΔthrB/pTrcA*H in Fed-Batch)
- 4.08 g/L (Escherichia coli W3110 ΔmetJΔmetIΔlysA/pTrcA*H in Flask)
- 9.75 g/L (Escherichia coli W3110 ΔmetJΔmetIΔlysA/pTrcA*H in Fed-Batch)
- 593.3 mg/L (Escherichia coli W3110 ΔmetJΔmetI (pTrcA*H) in Flask)
- 413.16 mg/L (Escherichia coli W3110 ΔmetJ (pTrcA*H) in Flask)
- 202.64 mg/L (Escherichia coli W3110 ΔmetJ/pTrcA* in Flask)
- 0.19 g/g of sustrate (Escherichia coli W3110 ΔmetJΔmetIΔlysA/pTrcA*H in Batch)
- 14.0 % (Escherichia coli W3110 ΔmetJΔmetIΔlysA/pTrcA*H in Fed-Batch)
- 0.02 g/g of sustrate (Escherichia coli W3110 ΔmetJΔmetIΔthrB/pTrcA*H in Batch)
- 0.03 g/g of sustrate (Escherichia coli W3110 ΔmetJΔmetI (pTrcA*H) in Batch)
- 0.2 g/g of sustrate (Escherichia coli W3110 ΔmetJΔmetIΔlysAΔthrB/pTrcA*H in Batch)
- 0.2 g/L/h (Escherichia coli W3110 ΔmetJΔmetIΔlysA/pTrcA*H in Fed-Batch)
pyruvate
- 194.5 mM (Escherichia coli TC38 in Batch)