Xueli Zhang, Kaemwich Jantama, J. C. Moore, K. T. Shanmugam & L. O. Ingram (2007). Production of l-alanine by metabolically engineered Escherichia coli. Applied Microbiology and Biotechnology.
Strains
- Escherichia coli DH5α/pTrc99A, pLyc184
- Escherichia coli SZ194
- Escherichia coli XZ105
- Escherichia coli XZ111
- Escherichia coli XZ112
- Escherichia coli XZ113
- Escherichia coli XZ115
- Escherichia coli XZ121
- Escherichia coli XZ123
- Escherichia coli XZ129
- Escherichia coli XZ130
- Escherichia coli XZ131
- Escherichia coli W
Metabolite production
-
L-alanine
-
Titer
- 836.0 mM (Escherichia coli XZ115 in Batch)
- 181.0 mM (Escherichia coli XZ111 in Batch)
- 832.0 mM (Escherichia coli XZ123 in Batch)
- 1069.0 mM (Escherichia coli XZ131 in Batch)
- 1279.0 mM (Escherichia coli XZ132 in Batch)
- 833.0 mM (Escherichia coli XZ129 in Batch)
- 847.0 mM (Escherichia coli XZ130 in Batch)
- 483.0 mM (Escherichia coli XZ113 in Batch) Substrate yield
- 0.93 g/g of sustrate (Escherichia coli XZ115 in Batch)
- 0.81 g/g of sustrate (Escherichia coli XZ111 in Batch)
- 0.95 g/g of sustrate (Escherichia coli XZ131 in Batch)
- 0.95 g/g of sustrate (Escherichia coli XZ132 in Batch)
- 0.93 g/g of sustrate (Escherichia coli XZ129 in Batch)
- 0.93 g/g of sustrate (Escherichia coli XZ130 in Batch)
- 93.0 % (Escherichia coli XZ123 in Batch)
- 86.0 % (Escherichia coli XZ113 in Batch)
-
succinate
-
Titer
- 2.0 mM (Escherichia coli XZ123 in Batch)
- 2.0 mM (Escherichia coli XZ129 in Batch)
- 2.0 mM (Escherichia coli XZ130 in Batch)
- 2.0 mM (Escherichia coli XZ131 in Batch)
- 2.0 mM (Escherichia coli XZ132 in Batch)
-
acetate
-
Titer
- 9.0 mM (Escherichia coli XZ123 in Batch)
- 8.0 mM (Escherichia coli XZ129 in Batch)
- 8.0 mM (Escherichia coli XZ130 in Batch)
- 7.0 mM (Escherichia coli XZ131 in Batch)
- 7.0 mM (Escherichia coli XZ132 in Batch)
- 7.0 mM (Escherichia coli XZ115 in Batch)
- 7.0 mM (Escherichia coli XZ113 in Batch)
-
(R)-lactate
-
Titer
- 5.0 mM (Escherichia coli XZ115 in Batch)
- 4.0 mM (Escherichia coli XZ113 in Batch)