Han Liu, Guochen Fang, Hui Wu, Zhimin Li & Qin Ye (2018). L‐Cysteine Production in Escherichia coli Based on Rational Metabolic Engineering and Modular Strategy. Biotechnology Journal.
Strains
- Escherichia coli SLH01
- Escherichia coli LH00
- Escherichia coli LH01
- Escherichia coli LH02
- Escherichia coli LH03
- Escherichia coli LH04
- Escherichia coli LH05
- Escherichia coli SLH02
- Escherichia coli LH06
- Escherichia coli JM109
Plasmids
Metabolite production
-
acetate
-
Titer
- 3.29 g/L (Escherichia coli LH03 in Flask)
-
L-cysteine
-
Titer
- 5.1 g/L (Escherichia coli LH06 in Fed-Batch)
- 620.9 mg/L (Escherichia coli LH06 in Flask)
- 547.3 mg/L (Escherichia coli LH05 in Flask)
- 45.8 mg/L (Escherichia coli LH03 in Flask)
- 190.3 mg/L (Escherichia coli LH03 in Flask)
- 492.0 mg/L (Escherichia coli LH04 in Flask) Substrate yield
- 0.06 g/g of sustrate (Escherichia coli LH06 in Flask)
- 0.053 g/g of sustrate (Escherichia coli LH05 in Flask)
- 0.004 g/g of sustrate (Escherichia coli LH03 in Flask)
- 0.018 g/g of sustrate (Escherichia coli LH03 in Flask)
- 0.047 g/g of sustrate (Escherichia coli LH04 in Flask)